A. Trichoderma harzianum
B. Meloidogyne incognita
C. Agrobacterium tumefaciens
D. Penicillium expansum
# DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by:
A. Centrifugation
B. Polymerase chain reaction
C. Electrophoresis
D. Restriction mapping
# Restriction endonucleases are enzymes which:
A. make cuts at specific positions within the DNA molecule
B. recognize a specific nucleotide sequence for binding of DNA ligase
C. restrict the action of the enzyme DNA polymerase
D. remove nucleotides from the ends of the DNA molecule
# A single strand of nucleic acid tagged with a radioactive molecule is called:
A. Vector
B. Selectable marker
C. Plasmid
D. Probe
# There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?
A. colon
B. coelom
C. coenzyme
D. coli
# The linking of antibioticc resistance gene with the plasmid vector became possible with:
A. DNA polymerase
B. Exonucleases
C. DNA ligase
D. Endonucleases
# Which vector can clone only a small fragment of DNA?
A. Bacterial artificial chromosome
B. Yeast artificial chromosome
C. Plasmid
D. Cosmid
# Which one of the following is used as vector for cloning genes into higher organisms?
A. Baculovirus
B. Salmonella typhimurium
C. Rhizopus nigricans
D. Retrovirus
# PCR and restriction fragment length polymorphism are the methods for:
A. study of enzymes
B. genetic transformation
C. DNA sequencing
D. genetic fingerprinting
# In vitro clonal propagation in plants is characterized by:
A. PCR and RAPD
B. Northern blotting
C. Electrophoresis
D. Microscopy
# Stirred tank bioreactors have been designed for:
A. addition of preservatives to the product
B. purification of the product
C. ensuring anaerobic conditions in the culture vessel
D. availability of oxygen throughout the process
# Which one is a true statement regarding DNA polymerase used in PCR?
A. It is used to ligate introduced DNA in recipient cells
B. It serves as a selectable marker
C. It is isolated from a virus
D. It remains active at high temperature
# Polyethylene glycol method is used for:
A. biodiesel production
B. seedless fruit production
C. energy production from sewage
D. gene transfer without a vector
# Gel electrophoresis is used for:
A. construction of recombinant DNA by joining with cloning vectors
B. isolation of DNA molecules
C. cutting of DNA into fragments
D. separation of DNA fragments according to their sizes
# An analysis of chromosomal DNA using the Southern hybridization technique does not use:
A. Electrophoresis
B. Blotting
C. Autoradiography
D. PCR
# Agarose extracted from sea weeds is used in:
A. Spectrophotometry
B. Tissue culture
C. PCR
D. Gel electrophoresis
# The colonies of recombinant bacteria appear white in contrast to blue colonies of non recombinant bacteria because of:
A. Non recombinant bacteria containing B-galactosidase
B. Insertional inactivation of alpha- galactosidase in non recombinant bacteria
C. Insertional inactivation of alpha- galactosidase in recombinant bacteria
D. Inactivation of glycosidase enzyme in recombinant bacteria
# For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
A. silver or platinum
B. platinum or zinc
C. silicon or platinum
D. gold or tungsten
# DNA fingerprinting was discovered by:
A. James Watson
B. Alec Jeffreys
C. Fredrick Sanger
D. Hargovind Khorana
# Plasmids are suitable vectors for gene cloning because:
A. these are small circular DNA molecules which can integrate with host chromosomal DNA
B. these are small circular DNA molecules with their own replication origin site
C. these can shuttle between prokaryotic and eukaryotic cells
D. these often carry antibiotic resistance genes
# The Ti plasmid, is often used for making transgenic plants. This plasmid is found in:
A. Azotobacter
B. Rhizobium of the roots of leguminous plants
C. Agrobacterium
D. Yeast as a 2 micrometer
# Advancement in genetic engineering has been possible due to the discovery of:
A. Oncogenes
B. Transposons
C. Restriction endonucleases
D. Exonucleases
# DNA is found in:
A. Chromosomes
B. Plastids
C. Mitochondria
D. All of the above
# Which of the following is related to genetic engineering?
A. Mutation
B. Plasmid
C. Plastid
D. Heterosis
# Dolly, the first cloned mammal, was produced by:
A. Parthenogenesis
B. Artificial fertilization
C. Replacement of zygote nucleus by somatic nucleus
D. In-vitro fertilization
# ELISA is used to detect viruses where the key reagent is:
A. alkaline phosphatase
B. catalase
C. DNA probe
D. RNAse
# Two bacteria found to be very useful in genetic engineering experiments are:
A. Nitrosomonas and Klebsiella
B. Escherichia and Agrobacterium
C. Nitrobacter and Azotobacter
D. Rhizobium and Diplococcus
# Restriction endonucleases:
A. are present in mammalian cells for degradation of DNA when the cell dies
B. are used in genetic engineering for ligating two DNA molecules
C. are used for in vitro DNA synthesis
D. are synthesized by bacteria as a part of their defence mechanism
# In transgenics, expression of transgene in target tissue is determined by:
A. enhancer
B. transgene
C. Promoter
D. reporter
# A dividing and undifferentiated mass of cells is called:
A. Callus
B. Embryo
C. Explant
D. Zygote
# Plasmid is:
A. Fragment of DNA which acts as vector
B. a fragment which joins two genes
C. mRNA which acts as carrier
D. autotrophic fragment
# Genetic engineering is possible because:
A. the phenomenon of transduction in bacteria is well understood.
B. we can see DNA by electron microscope
C. We can cut DNA at specific sites by endonucleases like DNAs- I
D. restriction endonucleases purified from bacteria can be used inverto
# In bacteria, Plasmid is:
A. extrachromosal material
B. Main DNA
C. non-functional DNA
D. repetitive gene
# Manipulation of DNA in genetic engineering became possible due to the discovery of:
A. restriction endonuclease
B. DNA ligase
C. transcription
D. primase
# The process of replication in plasmid DNA, other than initiation,is controlled by:
A. mitochondrial gene
B. bacterial gene
C. plasmid gene
D. None of the above
No comments:
Post a Comment